Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography

Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography

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Celangulin V, an insecticidal jerome brown jersey compound isolated from the root bark of Chinese bittersweet, can affect the digestive system of insects.However, the mechanism of how Celangulin V induces a series of symptoms is still unknown.In this study, affinity chromatography was conducted through coupling of Celangulin V-6-aminoacetic acid ester to the CNBr-activated Sepharose 4B.SDS-PAGE was used to analyze the collected fraction eluted by Celangulin V.Eight binding proteins (Zinc finger protein, Thioredoxin peroxidase (TPx), Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), SUMO E3 ligase RanBP2, click here Transmembrane protein 1, Actin, APN and V-ATPase) were obtained and identified by LC/Q-TOF-MS from the midgut of Mythimna separata larvae.

The potential of these proteins to serve as target proteins involved in the insecticidal activity of Celangulin V is discussed.